Semi Prep HPLC Column, NP Amino, 10um, 100A. 10mm ID x 50mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent NP Amino is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds that are often difficult to retain using conventional reversed-phase columns. Manufactured on high-purity silica with a propyl amino bonded phase, it provides excellent selectivity for carbohydrates, sugars, organic acids, and other polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC separations.

68040-SP50 is a semi-preparative HPLC column packed with 10 µm NP Amino particles featuring a 100 Å pore size in a 10 mm ID x 50 mm format. The larger internal diameter provides increased sample loading capacity compared to analytical columns while maintaining efficient chromatographic performance. The short 50 mm length is optimized for rapid method development, purification screening, and analytical-to-preparative method transfer. Typical operating flow rates are approximately 8–15 mL/min depending on mobile phase composition, sample load, and system configuration.

This column is particularly effective for sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other highly polar molecules. As with all amino phases, this column is suitable for both Normal Phase and HILIC applications when enhanced retention of polar analytes is required.

Key Benefits

  • NP Amino stationary phase

  • USP L8 equivalent chemistry

  • Propyl amino bonded phase

  • High-purity silica support

  • Suitable for Normal Phase chromatography

  • Suitable for HILIC applications

  • Enhanced retention of polar compounds

  • Excellent for sugars and carbohydrates

  • Low operating backpressure with 10 µm particles

  • Ideal for semi-preparative method development and purification scouting

Typical Applications

  • Semi-preparative chromatography

  • USP L8 methods

  • HILIC separations

  • Normal Phase chromatography

  • Sugar purification

  • Carbohydrate purification

  • Organic acid analysis and purification

  • Carboxylic acid separations

  • Pharmaceutical purification

  • Method scale-up from analytical columns

Specifications

  • Product Type: Semi Prep HPLC Column

  • Stationary Phase: NP Amino

  • USP Classification: L8

  • Chromatography Mode: Normal Phase / HILIC

  • Bonded Phase: Propyl Amino

  • Endcapping: Not Endcapped

  • Particle Size: 10 µm

  • Pore Size: 100 Å

  • Column Dimensions: 10 mm ID x 50 mm

  • Length: 50 mm

  • Typical Flow Rate: 8–15 mL/min

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Packed and shipped in Normal Phase solvents

  • Suitable for both Normal Phase and HILIC chromatographic modes

  • Excellent retention of sugars, carbohydrates, and organic acids

  • Short column length is ideal for rapid method development and purification scouting

  • Ideal for semi-preparative purification and analytical-to-prep method scale-up

  • Requires an HPLC system capable of supporting semi-preparative flow rates of approximately 8–15 mL/min

  • This is a Special Order Item


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